Peptide modification
Peptide modification:
C-end labeling technology
C-terminal amidation, aldehyde alcoholization, pNA, AMC, AFC, mercaptoethylamination, esterification, thiolipids, N-alkylation, etc
N-terminal labeling technology
Acetylation, HYNIC, biotin labeling, Br acetylation, chelated reaction (DOTA, DTPA combined), Dnp, formylation, tetradecyl, octadecyl, succinyl, palmitic, malic, fatty acidification
Fluorescent labeling modification
C-terminal modification
AFC, AMC, Dap (Dnp), Lys (Dye), pNA, Rh110
N-terminal modification
Bodpy-FL, Cy3, Cy5, Texas Red, 5-Tamra, 5-lodoacetamido fluorescein
Rhodamine 110 and Rhodamine B Luciferin, EDANS Dabcyl, dansyl
5-FAM, FITC, MCA, Rox, Sulforhodamine 101, 5-TAMRA
Fluorescent labeling such as FITC/Biotin/Fam
| Fluorescent modification Chinese name | N-terminal | N-end with linker |
| Biotin labeled peptides | Biotin- | Biotin-Ahx- |
| Fluorescein isothiocyanate | FITC- | FITC-Ahx- |
| 5-Carboxyfluorescein | 5-FAM- | 5-FAM-Ahx- |
| Dansulfoyl fluorescein | Dansyl- | Dansyl-Ahx- |
| 5-Carboxytetramethyl Rhodamine | TMR-（TAMRA-） | TMR-Ahx-（TAMRA-Ahx-） |
Fluorescence modification through LYS side chain amino linkage
| N-terminal peptide | Middle of peptide sequence | N-end with linker |
| Biotin labeled peptides | Biotin- | Peptide C-terminal |
| Lys(Biotin)- | -Lys(Biotin)- | -Lys(Biotin) |
| Lys(FITC)- | -Lys(FITC)- | -Lys(FITC) |
| Lys(5-FAM)- | -Lys(5-FAM)- | -Lys(5-FAM) |
| Lys(Dansyl)- | -Lys(Dansyl)- | -Lys(Dansyl) |
| Lys(TMR)- | -Lys(TMR)- | -Lys(TMR)- |
| Lys(Dnp)- | -Lys(Dnp)- | -Lys(Dnp) |
