Based on the same nuclear magnetic resonance phenomenon, people found the phenomenon of nuclear magnetic resonance and soon came into practical use. Chemists developed the nuclear magnetic resonance spectrum to analyze the molecular structure by taking advantage of the influence of molecular structure on the magnetic field around the hydrogen atom. Over time, the nuclear magnetic resonance technology has developed from the original one-dimensional hydrogen spectrum to 13C spectrum, two-dimensional nuclear magnetic resonance spectrum and other advanced spectra. The ability of nuclear magnetic resonance technology to analyze the molecular structure has become increasingly strong. On the other hand, medical experts use the principle of nuclear magnetic resonance (NMR) to detect the emitted electromagnetic waves in different structural environments within a substance based on the energy released. By applying a gradient magnetic field to detect the position and type of atomic nuclei that make up the object, the position and type of atomic nuclei that make up the object can be determined. Based on this, the internal structural image of the object can be drawn, which is called nuclear magnetic resonance imaging (NMRI). This technology has greatly promoted medicine The rapid development of neurophysiology and cognitive neuroscience.

  At present, the research field of nuclear magnetic resonance has won six Nobel Prizes in three fields (chemistry, physics and medicine), which is enough to illustrate the importance of this field and its derivative technologies.

The nuclear magnetic resonance technology is so powerful, so let's take Xinweichuang Biology to understand the role of nuclear magnetic resonance NMR in Drug development!

In the field of Drug development, drug analysis and detection are closely related to new drug research and development. The feature of drug analysis and detection is that it needs to consider the effectiveness and safety of the drug itself, mainly including quality control research on drugs such as APIs, preparations, excipients and intermediates, and in vivo drug analysis related to pharmacology, Drug metabolism process and clinical detection.

First of all, Xinweichuang will take you to understand what nuclear magnetic resonance NMR is

1、 NMR (Nuclear magnetic resonance spectroscopy)

It is an effective drug analysis and detection method that can determine the relative content of drugs in a mixture by comparing the characteristic spectral line strengths of hydrogen spectra. NMR is one of the most powerful tools for studying the absorption of radio frequency radiation by atomic nuclei, qualitatively analyzing the composition and structure of various organic and inorganic substances, and sometimes conducting quantitative analysis.

2、 Structural identification of drugs

2、 The NMR technology for drug structure identification is currently mainly used in drug structure identification, in vivo drug analysis and detection, in vitro drug analysis and detection, and metabolite analysis and detection. Its main goal is to improve sensitivity, such as the development of various multi pulse technologies.

New Vetrone Biotech can provide a full range of pharmaceutical analysis services, including method development and validation, analytical testing and release, stability research, large-scale separation and other services

3、 Quantitative and qualitative analysis methods for NMR

In quantitative analysis, the absolute content of a compound can be determined by comparing the peak area or peak height caused by protons on a specific functional group with the peak area caused by protons on a specific functional group in the reference standard.

When analyzing a mixture, relative comparisons can also be made using the absorption peak intensities generated by protons on the designated functional groups of each component, and then the relative content can be obtained.